Cloning, sequencing, and expression of ruvB and characterization of RuvB proteins from two distantly related thermophilic eubacteria.

@article{Tong1996CloningSA,
  title={Cloning, sequencing, and expression of ruvB and characterization of RuvB proteins from two distantly related thermophilic eubacteria.},
  author={Jiyun Tong and James G. Wetmur},
  journal={Journal of bacteriology},
  year={1996},
  volume={178 9},
  pages={2695-700}
}
The ruvB genes of the highly divergent thermophilic eubacteria Thermus thermophilus and Thermotoga maritima were cloned, sequenced, and expressed in Escherichia coli. Both thermostable RuvB proteins were purified to homogeneity. Like E. coli RuvB protein, both purified thermostable RuvB proteins showed strong double-stranded DNA-dependent ATPase activity at their temperature optima (> or = 70 degrees C). In the absence of ATP, T. thermophilus RuvB protein bound to linear double-stranded DNA… CONTINUE READING

From This Paper

Topics from this paper.
2 Citations
0 References
Similar Papers

Similar Papers

Loading similar papers…