Cloning, sequencing, and expression of equinatoxin II.

@article{Anderluh1996CloningSA,
  title={Cloning, sequencing, and expression of equinatoxin II.},
  author={Gregor Anderluh and J. Pungercar and B. Strukelj and Peter Ma{\vc}ek and F. Gubensek},
  journal={Biochemical and biophysical research communications},
  year={1996},
  volume={220 2},
  pages={437-42}
}
Equinatoxin II (EqtII), a basic protein of 179 amino acids lacking cysteine residues, is the most abundant cytolysin isolated from the sea anemone Actinia equina. Its mode of action is still poorly understood. In order to initiate further structure-function studies by protein engineering, cDNA library was prepared from the whole animal and hybridized with a PCR-derived probe, deduced from the EqtII primary structure. The longest positive clone of 899 bp was shown to encode a 214 residue… CONTINUE READING
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