Cloning, sequencing, and expression of RecA proteins from three distantly related thermophilic eubacteria.

@article{Wetmur1994CloningSA,
  title={Cloning, sequencing, and expression of RecA proteins from three distantly related thermophilic eubacteria.},
  author={James G. Wetmur and Diana M. L. Wong and Blanca Olivia Ca{\~n}edo Ortiz and Jian Tong and F. Reichert and David H. Gelfand},
  journal={The Journal of biological chemistry},
  year={1994},
  volume={269 41},
  pages={25928-35}
}
Sequences of the recA genes of the highly divergent thermopholic eubacteria Thermus aquaticus (and Thermus thermophilus), Thermotoga maritima, and Aquifex pyrophilus were determined from fragments derived by polymerase chain reaction (PCR) with degenerate primers and from inverse PCR products obtained using unique primers based on the fragment sequences. The source of the PCR products was verified by Southern hybridization. Complete PCR-derived recA genes were cloned into an expression vector… CONTINUE READING

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