Corpus ID: 28707640

Cloning, sequencing, and expression in Escherichia coli of the coat protein gene of a new potyvirus infection South African Passiflora.

@article{Brand1993CloningSA,
  title={Cloning, sequencing, and expression in Escherichia coli of the coat protein gene of a new potyvirus infection South African Passiflora.},
  author={R. Brand and J. Burger and E. Rybicki},
  journal={Archives of virology},
  year={1993},
  volume={128 1-2},
  pages={
          29-41
        }
}
  • R. Brand, J. Burger, E. Rybicki
  • Published 1993
  • Medicine
  • Archives of virology
  • Complementary DNA representing 1418 nucleotides (nt) of the 3'-poly(A)-proximal tract of the genomic RNA of a potyvirus causing woodiness disease in South African passion fruit, was cloned and sequenced. The sequence contained a single long open reading frame (ORF) of 1188 nt with no initiation codon, and a 3'-non-coding region (3'-NCR) of 230 nt followed by a poly-adenylate tract. Comparison of the ORF with other potyviral coat protein (CP) sequences led to the prediction that a 279 residue CP… CONTINUE READING
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