Cloning, sequencing, and disruption of the gene encoding sterol C-14 reductase in Saccharomyces cerevisiae.

@article{Lorenz1992CloningSA,
  title={Cloning, sequencing, and disruption of the gene encoding sterol C-14 reductase in Saccharomyces cerevisiae.},
  author={R. Lorenz and L. Parks},
  journal={DNA and cell biology},
  year={1992},
  volume={11 9},
  pages={
          685-92
        }
}
  • R. Lorenz, L. Parks
  • Published 1992
  • Biology, Medicine
  • DNA and cell biology
  • A sterol C-14 reductase (erg24-1) mutant of Saccharomyces cerevisiae was selected in a fen1, fen2, suppressor background on the basis of nystatin resistance and ignosterol (ergosta-8,14-dienol) production. The erg24-1 allele segregated genetically as a single, recessive gene. The wild-type ERG24 gene was cloned by complementation onto a 12-kb fragment from a yeast genomic library, and subsequently subcloned onto a 2.4-kb fragment. This was sequenced and found to contain an open reading frame of… CONTINUE READING
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    References

    SHOWING 1-9 OF 9 REFERENCES
    Physiological effects of fenpropimorph on wild-type Saccharomyces cerevisiae and fenpropimorph-resistant mutants.
    • 36
    • PDF
    High-efficiency transformation of yeast by electroporation.
    • 697
    The Biosynthesis of Ergosterol
    • 109
    Organization and expression of eucaryotic split genes coding for proteins.
    • 3,324