Cloning, sequencing, and disruption of the Bacillus subtilis sigma 28 gene.

@article{Helmann1988CloningSA,
  title={Cloning, sequencing, and disruption of the Bacillus subtilis sigma 28 gene.},
  author={J. D. Helmann and Lorenzo M{\'a}rquez and Michael J. Chamberlin},
  journal={Journal of bacteriology},
  year={1988},
  volume={170 4},
  pages={1568-74}
}
Bacillus subtilis contains multiple forms of RNA polymerase holoenzyme, distinguished by the presence of different specificity determinants known as sigma factors. The sigma 28 factor was initially purified as a unique transcriptional activity in vegetatively growing B. subtilis cells. Purification of the sigma 28 protein has allowed tryptic peptides to be prepared and sequenced. The sequence of one tryptic peptide fragment was used to prepare an oligonucleotide probe specific for the sigma 28… CONTINUE READING
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