Cloning, sequencing, and characterization of a heat- and alkali-stable type I pullulanase from Anaerobranca gottschalkii.

@article{Bertoldo2004CloningSA,
  title={Cloning, sequencing, and characterization of a heat- and alkali-stable type I pullulanase from Anaerobranca gottschalkii.},
  author={Costanzo Bertoldo and Martin Armbrecht and Fiona Becker and Thomas C Sch{\"a}fer and Garabed Antranikian and Wolfgang Liebl},
  journal={Applied and environmental microbiology},
  year={2004},
  volume={70 6},
  pages={3407-16}
}
The gene encoding a type I pullulanase was identified from the genome sequence of the anaerobic thermoalkaliphilic bacterium Anaerobranca gottschalkii. In addition, the homologous gene was isolated from a gene library of Anaerobranca horikoshii and sequenced. The proteins encoded by these two genes showed 39% amino acid sequence identity to the pullulanases from the thermophilic anaerobic bacteria Fervidobacterium pennivorans and Thermotoga maritima. The pullulanase gene from A. gottschalkii… CONTINUE READING
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