Cloning, sequence and expression in Escherichia coli of the gene encoding phosphofructokinase from Bacillus macquariensis.

  title={Cloning, sequence and expression in Escherichia coli of the gene encoding phosphofructokinase from Bacillus macquariensis.},
  author={Gerard J. A. Rouwendal and L. H. Zwiers and Emil J.H. Wolbert and Jan Springer and Hans Mooibroek and Henk J. Huizing},
  volume={171 1},
Cloning, Sequence, and Expression of the Phosphofructokinase Gene of Clostridium acetobutylicum ATCC 824 in Escherichia coli
The pfk gene encoding phosphofructokinase (Pfk) from the anaerobic bacterium Clostridium acetobutylicum ATCC 824 was cloned and sequenced and a coding region for a 319-aa protein homologous to Pfks from other organisms was revealed.


Cloning, sequencing, and expression in Escherichia coli of the gene coding for phosphofructokinase in Lactobacillus bulgaricus
A fragment of 1,185 bp containing the gene coding for phosphofructokinase in Lactobacillus bulgaricus has been cloned, sequenced, and expressed in Escherichia coli, suggesting that these enzymes have closely related structures despite their different regulatory properties.
Molecular cloning of a glucoamylase gene from a thermophilic Clostridium and kinetics of the cloned enzyme.
The deduced amino acid sequence suggests that a putative CGA precursor is modified and secreted like other bacterial lipoproteins in Clostridium sp.
Nucleotide sequence and high-level expression of the major Escherichia coli phosphofructokinase.
The gene for the major phosphofructokinase enzyme in Escherichia coli, pfkA, has been sequenced and comparison of the amino acid sequence showed that this enzyme is related to the Bacillus stearothermophilus and rabbit muscle enzymes, but is different from the second, minor phosphofructureokinase found in E. coli.
Molecular cloning of phosphofructokinase 1 gene from a thermophilic bacterium, Thermus thermophilus.
Structure and function of L-lactate dehydrogenases from thermophilic, mesophilic and psychrophilic bacteria, IX. Identification, isolation and nucleotide sequence of two L-lactate dehydrogenase genes of the psychrophilic bacterium Bacillus psychrosaccharolyticus.
Two genes encoding for L-lactate dehydrogenase (LDH) from the psychrophilic bacterium Bacillus psychrosaccharolyticus (DSM 6) were cloned and their nucleotide sequence determined using a pEMBL vector
Organization and nucleotide sequences of the Spiroplasma citri genes for ribosomal protein S2, elongation factor Ts, spiralin, phosphofructokinase, pyruvate kinase, and an unidentified protein
The gene for spiralin, the major membrane protein of the helical mollicute Spiroplasma citri, was cloned in Escherichia coli as a 5-kilobase-pair (kbp) DNA fragment. The complete nucleotide sequence
Gene for the ADP-ribosylatable elongation factor 2 from the extreme thermoacidophilic archaebacterium Sulfolobus acidocaldarius. Cloning, sequencing, comparative analysis.
Results indicate that, in contrast to all prokaryotic EF-2 genes studied previously, the gene of S. acidocaldarius is not located within the streptomycin operon but is transcribed separately.