Cloning, sequence analysis, and expression in Escherichia coli of the gene encoding an alpha-amino acid ester hydrolase from Acetobacter turbidans.

@article{PoldermanTijmes2002CloningSA,
  title={Cloning, sequence analysis, and expression in Escherichia coli of the gene encoding an alpha-amino acid ester hydrolase from Acetobacter turbidans.},
  author={Jolanda J. Polderman-Tijmes and Peter A. Jekel and Erik F. J. de Vries and Annet E J van Merode and Ren{\'e} Floris and J C van der Laan and Theo Sonke and Dick B. Janssen},
  journal={Applied and environmental microbiology},
  year={2002},
  volume={68 1},
  pages={211-8}
}
The alpha-amino acid ester hydrolase from Acetobacter turbidans ATCC 9325 is capable of hydrolyzing and synthesizing beta-lactam antibiotics, such as cephalexin and ampicillin. N-terminal amino acid sequencing of the purified alpha-amino acid ester hydrolase allowed cloning and genetic characterization of the corresponding gene from an A. turbidans genomic library. The gene, designated aehA, encodes a polypeptide with a molecular weight of 72,000. Comparison of the determined N-terminal… CONTINUE READING
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