Cloning, purification and characterisation of Staphylococcus warneri lipase 2.

@article{Kampen2001CloningPA,
  title={Cloning, purification and characterisation of Staphylococcus warneri lipase 2.},
  author={Muriel D. van Kampen and Ralf Rosenstein and Friedrich G{\"o}tz and Maarten R. Egmond},
  journal={Biochimica et biophysica acta},
  year={2001},
  volume={1544 1-2},
  pages={229-41}
}
A gene encoding an extracellular lipase was identified in Staphylococcus warneri 863. The deduced lipase is organised as a prepro-protein and has significant similarity to other staphylococcal lipases. The mature part of the lipase was expressed with an N-terminal histidine tag in Escherichia coli, purified and biochemically characterised. The results show that the purified lipase (named SWL2) combines the properties of the staphylococcal lipases characterised so far. It has both a high… CONTINUE READING

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