Cloning, purification, crystallization and preliminary structural studies of penicillin V acylase from Bacillus subtilis.

@article{Rathinaswamy2005CloningPC,
  title={Cloning, purification, crystallization and preliminary structural studies of penicillin V acylase from Bacillus subtilis.},
  author={Priya Rathinaswamy and Archana Vishnu Pundle and Asmita A Prabhune and H. Sivaraman and James A Brannigan and Guy G. Dodson and C. G. Suresh},
  journal={Acta crystallographica. Section F, Structural biology and crystallization communications},
  year={2005},
  volume={61 Pt 7},
  pages={680-3}
}
Penicillin acylase proteins are amidohydrolase enzymes that cleave penicillins at the amide bond connecting the side chain to their beta-lactam nucleus. An unannotated protein from Bacillus subtilis has been expressed in Escherichia coli, purified and confirmed to possess penicillin V acylase activity. The protein was crystallized using the hanging-drop… CONTINUE READING