Cloning, purification, and characterization of galactomannan-degrading enzymes from Myceliophthora thermophila

@article{Dotsenko2012CloningPA,
  title={Cloning, purification, and characterization of galactomannan-degrading enzymes from Myceliophthora thermophila},
  author={Gleb S Dotsenko and Margarita V. Semenova and Olga A Sinitsyna and Sandra W. A. Hinz and Jan Wery and Ivan N Zorov and Elena Kondratieva and Arkady P. Sinitsyn},
  journal={Biochemistry (Moscow)},
  year={2012},
  volume={77},
  pages={1303-1311}
}
Genes of β-mannosidase 97 kDa, GH family 2 (bMann9), β-mannanase 48 kDa, GH family 5 (bMan2), and α-galactosidase 60 kDa, GH family 27 (aGal1) encoding galactomannan-degrading glycoside hydrolases of Myceliophthora thermophila C1 were successfully cloned, and the recombinant enzymes were purified to homogeneity and characterized. bMann9 displays only exo-mannosidase activity, the K m and k cat values are 0.4 mM and 15 sec−1 for p-nitrophenyl-β-D-mannopyranoside, and the optimal pH and… CONTINUE READING
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References

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Protein Sequencing Protocols, Humana Press, Totowa

  • B. E. Smith
  • 1997

Carbohydrate Analysis: A Practical Approach (2nd Edn.)

  • M. F. Chaplin, J. F. Kennedy
  • 1994
2 Excerpts

Advances in Carbohydrate Chemistry and Biochemistry (Tipson

  • B. V. McCleary, N. K. Matheson
  • 1986
1 Excerpt

Introduction to Modern Liquid Chromatography

  • L. R. Snyder, J. J. Kirkland
  • 1979
1 Excerpt

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