Cloning, purification, and biochemical characterization of the pneumococcal bacteriophage Cp-1 lysin.

@article{Garcia1987CloningPA,
  title={Cloning, purification, and biochemical characterization of the pneumococcal bacteriophage Cp-1 lysin.},
  author={J{\'e}ssica Leite Garcia and Ernesto Garcia and A Arrar{\'a}s and Pedro Garc{\'i}a and Concepci{\'o}n Ronda and R. Vara Lopez},
  journal={Journal of virology},
  year={1987},
  volume={61 8},
  pages={2573-80}
}
Cp-1, a small virulent bacteriophage infecting Streptococcus pneumoniae, encodes its own lytic enzyme (CPL). A fragment of Cp-1 DNA containing the gene cpl coding for CPL was cloned and expressed in high amounts in Escherichia coli. CPL was purified to electrophoretic homogeneity by using affinity chromatography on choline-Sepharose (T. Briese and R. Hakenbeck, Eur. J. Biochem. 146:417-427, 1985), and the enzyme showing a Mr of 39,000 was characterized as a muramidase. This muramidase required… CONTINUE READING
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