Cloning, overexpression and characterization of a new oligoalginate lyase from a marine bacterium, Shewanella sp.

@article{Wang2014CloningOA,
  title={Cloning, overexpression and characterization of a new oligoalginate lyase from a marine bacterium, Shewanella sp.},
  author={Linna Wang and Shangyong Li and Wengong Yu and Qianhong Gong},
  journal={Biotechnology Letters},
  year={2014},
  volume={37},
  pages={665-671}
}
Is to report an oligoalginate lyase with high enzymatic activity and high-level expression. Using site-finding PCR and degenerate PCR, a gene (designated oalS17) encoding a new oligoalginate lyase was cloned from Shewanella sp. Kz7 and expressed in Escherichia coli. The gene consisted of 2,292 bp with deduced amino acid size of 763 including a putative signal peptide of 44 amino acid residues belonging to polysaccharide lyase (PL) family 17. The recombinant protein was most active at 50 °C and… CONTINUE READING