Cloning, overexpression, and genomic mapping of the 14-kDa subunit of human replication protein A.

@article{Umbricht1993CloningOA,
  title={Cloning, overexpression, and genomic mapping of the 14-kDa subunit of human replication protein A.},
  author={Christopher B Umbricht and Lorne F. Erdile and Ethylin Wang Jabs and Thomas B Kelly},
  journal={The Journal of biological chemistry},
  year={1993},
  volume={268 9},
  pages={
          6131-8
        }
}
Replication protein A (RPA) is a three-subunit protein that plays a central role in eukaryotic DNA replication, recombination, and repair. We have previously reported the cloning and bacterial expression of the 70- and 32-kDa subunits of human RPA (hRPA). We have now cloned the 14-kDa subunit (hRPA3) from a HeLa cell cDNA library. The hRPA3 cDNA is a 692-base pair sequence that contains an open reading frame encoding a protein of 121 amino acids with a calculated molecular mass of 13.6 kDa. The… CONTINUE READING