Cloning, nucleotide sequence, expression, and chromosomal location of ldh, the gene encoding L-(+)-lactate dehydrogenase, from Lactococcus lactis.

@article{Llanos1992CloningNS,
  title={Cloning, nucleotide sequence, expression, and chromosomal location of ldh, the gene encoding L-(+)-lactate dehydrogenase, from Lactococcus lactis.},
  author={R Mart{\'i}nez Llanos and Alan J. Hillier and B. E. Davidson},
  journal={Journal of bacteriology},
  year={1992},
  volume={174 21},
  pages={
          6956-64
        }
}
A gene (designated ldh) that encodes fructose-1,6-bisphosphate-activated L-(+)-lactate dehydrogenase was cloned from Lactococcus lactis subsp. lactis. Plasmids containing ldh conferred fructose-1,6-bisphosphate-activated L-(+)-lactate dehydrogenase activity on Escherichia coli cells. This activity was conferred only when a promoter had been introduced into the plasmid to express the cloned ldh. The nucleotide sequence of ldh predicted a chain length of 324 amino acids and a subunit molecular… CONTINUE READING

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