Cloning, mapping, and characterization of the Escherichia coli prc gene, which is involved in C-terminal processing of penicillin-binding protein 3.

@article{Hara1991CloningMA,
  title={Cloning, mapping, and characterization of the Escherichia coli prc gene, which is involved in C-terminal processing of penicillin-binding protein 3.},
  author={Hiroshi Hara and Yoko Yamamoto and Atsushi Higashitani and Hideho Suzuki and Yosuke Nishimura},
  journal={Journal of bacteriology},
  year={1991},
  volume={173 15},
  pages={4799-813}
}
The prc gene, which is involved in cleavage of the C-terminal peptide from the precursor form of penicillin-binding protein 3 (PBP 3) of Escherichia coli, was cloned and mapped at 40.4 min on the chromosome. The gene product was identified as a protein of about 80 kDa in maxicell and in vitro systems. Fractionation of the maxicells producing the product suggested that the product was associated with the periplasmic side of the cytoplasmic membrane. This was consistent with the notion that the C… CONTINUE READING

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