Cloning, expression and some properties of alpha-carbonic anhydrase from Helicobacter pylori.

@article{Chirica2001CloningEA,
  title={Cloning, expression and some properties of alpha-carbonic anhydrase from Helicobacter pylori.},
  author={Laura C Chirica and Bj{\"o}rn Elleby and Sven Lindskog},
  journal={Biochimica et biophysica acta},
  year={2001},
  volume={1544 1-2},
  pages={55-63}
}
The alpha-carbonic anhydrase gene from Helicobacter pylori strain 26695 has been cloned and sequenced. The full-length protein appears to be toxic to Escherichia coli, so we prepared a modified form of the gene lacking a part that presumably encodes a cleavable signal peptide. This truncated gene could be expressed in E. coli yielding an active enzyme comprising 229 amino acid residues. The amino acid sequence shows 36% identity with that of the enzyme from Neisseria gonorrhoeae and 28% with… CONTINUE READING
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