Cloning, expression and purification of Microcystis viridis lectin in Escherichia coli.

@article{Li2011CloningEA,
  title={Cloning, expression and purification of Microcystis viridis lectin in Escherichia coli.},
  author={Yuqin Li and Xiaoxia Liao and G. Lily Chen and YeeLeng Yap and Xuewu Zhang},
  journal={Molecular biotechnology},
  year={2011},
  volume={47 2},
  pages={105-10}
}
Microcystis viridis lectin (MVL), a sugar-binding protein originally isolated from freshwater blue-green algae Microcystis viridis, has been reported to have potent anti-HIV activity. In this paper, we described the expression and purification of recombinant-MVL (R-MVL) gene in E. coli. The results demonstrated that the R-MVL in shake flask cultures was… CONTINUE READING