• DOI:10.1007/s00792-012-0442-3
Corpus ID: 12785590

Cloning, expression and characterization of a halotolerant esterase from a marine bacterium Pelagibacterium halotolerans B2T

@article{Jiang2012CloningEA,
  title={Cloning, expression and characterization of a halotolerant esterase from a marine bacterium Pelagibacterium halotolerans B2T},
  author={Xiawei Jiang and Yingyi Huo and H. Cheng and Xinqi Zhang and Xufen Zhu and Min Wu},
  journal={Extremophiles},
  year={2012},
  volume={16},
  pages={427-435}
}

Xiawei Jiang, Yingyi Huo, +3 authors Min Wu

Published 2012

Biology, Medicine

Extremophiles

An esterase PE10 (279 aa) from Pelagibacterium halotolerans B2T was cloned and overexpressed in Escherichia coli Rosetta in a soluble form. The deduced protein was 29.91 kDa and the phylogenetic analysis of the deduced amino acids sequence showed it represented a new family of lipolytic enzymes. The recombinant protein was purified by Ni–NTA affinity chromatography column and the characterization showed its optimal temperature and pH were 45 °C and pH 7.5, respectively. Substrate specificity… CONTINUE READING

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