Cloning, expression and characterization of a 46.5-kDa metallopeptidase from Bacillus halodurans H4 sharing properties with the pitrilysin family.

@article{Dabonn2005CloningEA,
  title={Cloning, expression and characterization of a 46.5-kDa metallopeptidase from Bacillus halodurans H4 sharing properties with the pitrilysin family.},
  author={Soumaila Dabonn{\'e} and Claire Moallic and J Sine and S{\'e}bastien Lamine Niamk{\'e} and Michel Dion and Bernard Colas},
  journal={Biochimica et biophysica acta},
  year={2005},
  volume={1725 1},
  pages={136-43}
}
A 1242 base pair DNA fragment from Bacillus halodurans H4 isolated from alkaline sediments of Lake Bogoria (Kenya) coding for a potential protease was cloned and sequenced. The hexa-histidine-tagged enzyme was overexpressed in Escherichia coli and was purified in one step by immobilized-metal affinity chromatography (IMAC) on Ni-NTA resin. The protease… CONTINUE READING