Cloning, expression and characterization of a family 48 exocellulase, Cel48A, from Thermobifida fusca.

@article{Irwin2000CloningEA,
  title={Cloning, expression and characterization of a family 48 exocellulase, Cel48A, from Thermobifida fusca.},
  author={Diana C. Irwin and Shuang Zhang and David B. Wilson},
  journal={European journal of biochemistry},
  year={2000},
  volume={267 16},
  pages={4988-97}
}
The gene for a 104-kDa exocellulase, Cel48A, formerly E6, was cloned from Thermobifida fusca into Escherichia coli and Streptomyces lividans. The DNA sequence revealed a type II cellulose-binding domain at the N-terminus, followed by a FNIII-like domain and ending with a glycosyl hydrolase Family 48 catalytic domain. The enzyme and catalytic domain alone were each expressed in and purified from S. lividans and had very low catalytic activity on swollen cellulose, carboxymethyl cellulose… CONTINUE READING

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