Cloning, expression and characterisation of FKB-6, the sole large TPR-containing immunophilin from C. elegans.

@article{Richardson2007CloningEA,
  title={Cloning, expression and characterisation of FKB-6, the sole large TPR-containing immunophilin from C. elegans.},
  author={Julia M Richardson and Jacqueline Dornan and Monluedee Opamawutthikul and Sandra Bruce and Antony P. Page and Malcolm D. Walkinshaw},
  journal={Biochemical and biophysical research communications},
  year={2007},
  volume={360 3},
  pages={566-72}
}
We have cloned, expressed, purified and characterised ceFKB-6, the only large tetratricopeptide repeat motif-containing immunophilin in Caenorhabditis elegans which is similar to the human orthologues FKBP51 and FKBP52. It shows increased peptidyl prolyl isomerase activity, the measured k(cat)/K(m) of 1.3 x 10(6) M(-1) s(-1)is twofold greater than that of hFKBP12 and hFKBP51. NMR studies of the interaction between FKB-6 and the C-terminal DAF-21 pentapeptide MEEVD show interactions consistent… CONTINUE READING