Cloning, expression and bioinformatics analysis of ATP sulfurylase from Acidithiobacillus ferrooxidans ATCC 23270 in Escherichia coli

Michael L. Jaramillo; M. Abanto; Ruth L Quispe; J. Calderón; L. D. Del Valle; Miguel Talledo; P. Ramírez

DOI:10.6026/97320630008695

Corpus ID: 15328815

Cloning, expression and bioinformatics analysis of ATP sulfurylase from Acidithiobacillus ferrooxidans ATCC 23270 in Escherichia coli

@article{Jaramillo2012CloningEA,
  title={Cloning, expression and bioinformatics analysis of ATP sulfurylase from Acidithiobacillus ferrooxidans ATCC 23270 in Escherichia coli},
  author={Michael L. Jaramillo and M. Abanto and Ruth L Quispe and J. Calder{\'o}n and L. D. Del Valle and Miguel Talledo and P. Ram{\'i}rez},
  journal={Bioinformation},
  year={2012},
  volume={8},
  pages={695 - 704}
}

Michael L. Jaramillo, M. Abanto, +4 authors P. Ramírez

Published 2012

Biology, Medicine

Bioinformation

Molecular studies of enzymes involved in sulfite oxidation in Acidithiobacillus ferrooxidans have not yet been developed, especially in the ATP sulfurylase (ATPS) of these acidophilus tiobacilli that have importance in biomining. This enzyme synthesizes ATP and sulfate from adenosine phosphosulfate (APS) and pyrophosphate (PPi), final stage of the sulfite oxidation by these organisms in order to obtain energy. The atpS gene (1674 bp) encoding the ATPS from Acidithiobacillus ferrooxidans ATCC… CONTINUE READING

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