Cloning, expression, and up-regulation of inducible rat prostaglandin e synthase during lipopolysaccharide-induced pyresis and adjuvant-induced arthritis.

@article{Mancini2001CloningEA,
  title={Cloning, expression, and up-regulation of inducible rat prostaglandin e synthase during lipopolysaccharide-induced pyresis and adjuvant-induced arthritis.},
  author={Joseph A. Mancini and Katherine A Blood and Jocelyne Guay and Robert Gordon and David Claveau and C C Chan and Denis Riendeau},
  journal={The Journal of biological chemistry},
  year={2001},
  volume={276 6},
  pages={4469-75}
}
We have cloned and expressed the inducible form of prostaglandin (PG) E synthase from rat and characterized its regulation of expression in several tissues after in vivo lipopoylsaccharide (LPS) challenge. The rat PGE synthase is 80% identical to the human enzyme at the amino acid level and catalyzes the conversion of PGH(2) to PGE(2) when overexpressed in Chinese hamster ovary K1 (CHO-K1) cells. PGE synthase activity was measured using [(3)H]PGH(2) as substrate and stannous chloride to… CONTINUE READING
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