Cloning, expression, and sequence analysis of the Haemophilus influenzae type b strain M43p+ pilin gene.

@article{Gilsdorf1990CloningEA,
  title={Cloning, expression, and sequence analysis of the Haemophilus influenzae type b strain M43p+ pilin gene.},
  author={Janet R. Gilsdorf and Carl F. Marrs and Kirk W. McCrea and Larry J. Forney},
  journal={Infection and immunity},
  year={1990},
  volume={58 4},
  pages={1065-72}
}
By using antiserum against Haemophilus influenzae type b (Hib) strain M43p+ denatured pilin, we screened a genomic library of Hib strain M43p+ and identified a clone that expressed pilin, but not assembled pili, on its surface. Southern blot analysis revealed the presence of one structural gene, which was also present in strain M42p-, a nonpiliated variant. Five exonuclease III deletion mutants, two of which had deletions that extended into the structural gene and failed to express pilin, were… CONTINUE READING