Cloning, expression, and localization of a rat brain high-affinity glycine transporter.

  title={Cloning, expression, and localization of a rat brain high-affinity glycine transporter.},
  author={John Guastella and Nicholas C Brecha and C Weigmann and Henry A. Lester and Norman Davidson},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={89 15},
A cDNA clone encoding a glycine transporter has been isolated from rat brain by a combined PCR and plaque-hybridization strategy. mRNA synthesized from this clone (designated GLYT1) directs the expression of sodium- and chloride-dependent, high-affinity uptake of [3H]glycine by Xenopus oocytes. [3H]Glycine transport mediated by clone GLYT1 is blocked by sarcosine but is not blocked by methyl-aminoisobutyric acid or L-alanine, a substrate specificity similar to that described for a previously… CONTINUE READING
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