Cloning, expression, and characterization of cadmium and manganese uptake genes from Lactobacillus plantarum.

@article{Hao1999CloningEA,
  title={Cloning, expression, and characterization of cadmium and manganese uptake genes from Lactobacillus plantarum.},
  author={Zhiqi Hao and Shuangya Chen and David B. Wilson},
  journal={Applied and environmental microbiology},
  year={1999},
  volume={65 11},
  pages={4746-52}
}
An Mn(2+) and Cd(2+) uptake gene, mntA, was cloned from Lactobacillus plantarum ATCC 14917 into Escherichia coli. Its expression conferred on E. coli cells increased Cd(2+) sensitivity as well as energy-dependent Cd(2+) uptake activity. Both transcription and translation of mntA were induced by Mn(2+) starvation in L. plantarum, as indicated by reverse transcriptase PCR and immunoblotting. Two Cd(2+) uptake systems have been identified in L. plantarum: one is a high-affinity Mn(2+) and Cd(2… CONTINUE READING

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