Cloning, expression, and characterization of a single-domain antibody fragment with affinity for 15-acetyl-deoxynivalenol.

@article{Doyle2008CloningEA,
  title={Cloning, expression, and characterization of a single-domain antibody fragment with affinity for 15-acetyl-deoxynivalenol.},
  author={Patrick J Doyle and Mehdi Arbabi-Ghahroudi and Nathalie Gaudette and Gordon S Furzer and Marc E. Savard and Steve C Gleddie and Michael D. McLean and Colin R MacKenzie and J. Christopher Hall},
  journal={Molecular immunology},
  year={2008},
  volume={45 14},
  pages={3703-13}
}
A single-domain variable heavy chain (V(H)H) antibody fragment specific to the mycotoxin 15-acetyldeoxynivalenol (15-AcDON) was obtained after immunization of a llama (Llama glama) with the protein conjugate 15-DON-BSA plus TiterMax Classic adjuvant. After confirmation of a polyclonal response to DON toxin in both conventional (cIgG) and heavy chain antibody (HCAb) fractions, a V(H)H library was constructed from amplified cDNA by nested PCR. V(H)H fragments with binding affinity for the… CONTINUE READING

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