Cloning, expression, and characterization of a D-psicose 3-epimerase from Clostridium cellulolyticum H10.

Abstract

The noncharacterized protein ACL75304 encoded by the gene Ccel_0941 from Clostridium cellulolyticum H10 (ATCC 35319), previously proposed as the xylose isomerase domain protein TIM barrel, was cloned and expressed in Escherichia coli . The expressed enzyme was purified by nickel-affinity chromatography with electrophoretic homogeneity and then characterized… (More)
DOI: 10.1021/jf201356q

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