Cloning, characterization and expression of the D2 dopamine receptor from the tilapia pituitary.

@article{LevaviSivan2005CloningCA,
  title={Cloning, characterization and expression of the D2 dopamine receptor from the tilapia pituitary.},
  author={Berta Levavi-Sivan and Joseph Aizen and Ayelet Avitan},
  journal={Molecular and cellular endocrinology},
  year={2005},
  volume={236 1-2},
  pages={17-30}
}
A full-length cDNA encoding a dopamine receptor (DA-R) was obtained from the pituitary of tilapia (ta). This cDNA encodes a protein of 469 amino acids that exhibits the typical arrangement of GPCR. The taDA-R shows high similarity to the DA-Rs of mullet and fugu, and over 70% similarity to Xenopus, mouse and turkey D2 DA-Rs. Northern blot analysis revealed transcript for a single transcript in the pituitary, of approximately 3 kb. In a Southern analysis, the tilapia probe recognized specific… CONTINUE READING