Cloning, characterization and expression of a new cry1Ab gene from Bacillus thuringiensisWB9

@article{Huang2004CloningCA,
  title={Cloning, characterization and expression of a new cry1Ab gene from Bacillus thuringiensisWB9},
  author={Zhipeng Huang and Chunhong Guan and Xiong Guan},
  journal={Biotechnology Letters},
  year={2004},
  volume={26},
  pages={1557-1561}
}
A new cry1Ab-type gene, cry1Ab17, was cloned from Bacillus thuringiensis WB9 by PCR. Nucleotide sequence indicated that the open reading frames (ORFs) consists of 3471 bases and encodes a protein of 1156 residues with a calculated molecular weight of 130.5 kDa and an pI value of 5.04. Homology comparison revealed that the deduced amino acid sequence of Cry1Ab17 had 95.4% to 99.7% identity with those of the known Cry1Ab proteins. The Cry1Ab17 was one residue longer than the known Cry1Ab (except… CONTINUE READING

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