Cloning, characterization and expression analysis of nucleotide metabolism-related genes of mycobacteriophage L5.


The genomes of mycobacteriophages of the L5 family, which includes the lytic phage D29, contain several genes putatively linked to nucleotide-metabolizing functions. Two such genes, 48 and 50, encoding thymidylate synthase and ribonucleotide reductase (RNR), respectively, were overexpressed in Escherichia coli and the recombinant proteins were biochemically… (More)
DOI: 10.1111/j.1574-6968.2007.01047.x