Cloning, characterization, and use in strain improvement of the Cephalosporium acremonium gene cefG encoding acetyl transferase

@article{Mathison1993CloningCA,
  title={Cloning, characterization, and use in strain improvement of the Cephalosporium acremonium gene cefG encoding acetyl transferase},
  author={Lorilee Mathison and Charles Soliday and Tony Stepan and Teri Aldrich and John Rambosek},
  journal={Current Genetics},
  year={1993},
  volume={23},
  pages={33-41}
}
A long open reading frame (ORF) closely linked to the Cephalosporium acremonium gene cefEF was identified by DNA sequencing. The cefEF gene encodes the enzyme involved in cephalosporin C (CPC) biosynthesis known as expandase/hydroxylase. Complementation of a C. acremonium cefG mutant, as well as expression of the gene in Aspergillus niger, showed this ORF… CONTINUE READING