Cloning, characterization, and molecular application of a beta-agarase gene from Vibrio sp. strain V134.

@article{Zhang2007CloningCA,
  title={Cloning, characterization, and molecular application of a beta-agarase gene from Vibrio sp. strain V134.},
  author={Wei-Wei Zhang and Li Sun},
  journal={Applied and environmental microbiology},
  year={2007},
  volume={73 9},
  pages={2825-31}
}
V134, a marine isolate of the Vibrio genus, was found to produce a new beta-agarase of the GH16 family. The relevant agarase gene agaV was cloned from V134 and conditionally expressed in Escherichia coli. Enzyme activity analysis revealed that the optimum temperature and pH for the purified recombinant agarase were around 40 degrees C and 7.0. AgaV was demonstrated to be useful in two aspects: first, as an agarolytic enzyme, the purified recombinant AgaV could be employed in the recovery of DNA… CONTINUE READING

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