Corpus ID: 45663880

Cloning, Expression and Identification of Matrix Protein Gene of Wheat Rosette Stunt Virus (WRSV).

@article{Ye1998CloningEA,
  title={Cloning, Expression and Identification of Matrix Protein Gene of Wheat Rosette Stunt Virus (WRSV).},
  author={Yong-Jun Ye and Z. Gong},
  journal={Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica},
  year={1998},
  volume={30 5},
  pages={
          520-524
        }
}
  • Yong-Jun Ye, Z. Gong
  • Published 1998
  • Biology, Medicine
  • Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica
cDNA fragment located downstream of the WRSV NS protein gene was sequenced. Following an 80 nt untranslated region at the 5 'terminus, there is a 453 nt open reading frame(ORF) encoding a 17 kD protein, which was allowed to be expressed in E. coli using bacterial expression vector pGEX-3X and produced a 43 kD fusion protein. The result of Western blot showed that the fusion protein was able to react strongly with antibody raised against the purified WRSV particles. According to the similarities… Expand
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