Cloning, Expression, and Purification of Recombinant Uricase Enzyme from Pseudomonas aeruginosa Ps43 Using Escherichia coli.

@article{Shaaban2015CloningEA,
  title={Cloning, Expression, and Purification of Recombinant Uricase Enzyme from Pseudomonas aeruginosa Ps43 Using Escherichia coli.},
  author={Mona Ibrahim Shaaban and Eman Salama Ahmed Abdelmegeed and Youssif Mohammed Ali},
  journal={Journal of microbiology and biotechnology},
  year={2015},
  volume={25 6},
  pages={
          887-92
        }
}
Uricase is an important microbial enzyme that can be used in the clinical treatment of gout, hyperuricemia, and tumor lysis syndrome. A total of 127 clinical isolates of Pseudomonas aeruginosa were tested for uricase production. A Pseudomonas strain named Ps43 showed the highest level of native uricase enzyme expression. The open reading frame of the uricase enzyme was amplified from Ps43 and cloned into the expression vector pRSET-B. Uricase was expressed using E. coli BL21 (DE3). The ORF was… CONTINUE READING
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