Cloning, Expression, and Enzyme Characterization of an Acid Heat-Stable Phytase from Aspergillus fumigatus WY-2

@article{Wang2006CloningEA,
  title={Cloning, Expression, and Enzyme Characterization of an Acid Heat-Stable Phytase from Aspergillus fumigatus WY-2},
  author={Yanli Wang and Xiaorong Gao and Q. Z. Su and Wei Wu and Lijia An},
  journal={Current Microbiology},
  year={2006},
  volume={55},
  pages={65-70}
}
A novel thermostable phytase gene was cloned from Aspergillus fumigatus WY-2. It was 1459 bp in size and encoded a polypeptide of 465 amino acids. The gene was expressed in Pichia pastoris GS115 as an extracellular enzyme. The expressed enzyme was purified to homogeneity and biochemically characterized. The purified enzyme had a specific activity of 51 U/mg with an approximate molecular mass of 88 kDa. The optimum pH and temperature for activity were pH 5.5 and 55°C, respectively. After… CONTINUE READING
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Showing 1-10 of 23 references

Aspergillus fumigatus phytase, A

  • M Wyss, L Pasamontes, +5 authors molds van Loon APGM Comparison of the thermostability pr from
  • niger phytase and A. niger pH2.5 acid phosphatase…
  • 2007
1 Excerpt

Production studies and catalytic properties of phytases (myo-inositolhexakisphosphate phosphohydrolases): an overview

  • P Vats, UC Banerjee
  • Enzyme Microbiol Technol
  • 2004
1 Excerpt

In vitro properties of phytases from various microbial origins

  • O Simon, F Igbasan
  • Int J Food Sci Technol
  • 2002
1 Excerpt

Enzymatic characteristics of phytases as they relate to their use in animal feeds

  • DD Maenz
  • 2001

Biochemical characterization of fungal phytases ( myo - inositol hexakisphosphate phos - phohydrolases ) : catalytic properties

  • M Wyss, R Brugger, +4 authors van Loon APGM
  • Appl Environ Microbiol
  • 1999

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