Cloning, Biochemical Characterization and Inhibition of Alanine racemase from Streptococcus iniae

  title={Cloning, Biochemical Characterization and Inhibition of Alanine racemase from Streptococcus iniae},
  author={M. Muhammad and Yangyang Li and Siyu Gong and Yanmin Shi and J. Ju and Baohua Zhao and D. Liu},
Streptococcus iniae is a pathogenic and zoonotic bacteria that impacted high mortality to many fish species, as well as capable of causing serious disease to humans. Alanine racemase (Alr, EC is a pyridoxal-5′-phosphate (PLP)-containing homodimeric enzyme that catalyzes the racemization of L-alanine and D-alanine. In this study, we purified alanine racemase from the pathogenic strain of S. iniae, determined its biochemical characteristics and inhibitors. The alr gene has an open… Expand
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