Cloned transgenic cattle produce milk with higher levels of β-casein and κ-casein

@article{Brophy2003ClonedTC,
  title={Cloned transgenic cattle produce milk with higher levels of β-casein and κ-casein},
  author={Brigid Brophy and G. Smolenski and Thomas Wheeler and D. Wells and P. L'Huillier and G. Laible},
  journal={Nature Biotechnology},
  year={2003},
  volume={21},
  pages={157-162}
}
To enhance milk composition and milk processing efficiency by increasing the casein concentration in milk, we have introduced additional copies of the genes encoding bovine β- and κ-casein (CSN2 and CSN3, respectively) into female bovine fibroblasts. Nuclear transfer with four independent donor cell lines resulted in the production of 11 transgenic calves. The analysis of hormonally induced milk showed substantial expression and secretion of the transgene-derived caseins into milk. Nine cows… Expand
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