Cloned pigs produced by nuclear transfer from adult somatic cells

@article{Polejaeva2000ClonedPP,
  title={Cloned pigs produced by nuclear transfer from adult somatic cells},
  author={Irina A. Polejaeva and Shu-Hung Chen and Todd D. Vaught and Raymond L. Page and J. I. Mullins and Suyapa F Ball and Yifan Dai and Jeremy Boone and Shawn Walker and David Ayares and Alan Colman and Keith Henry Stockman Campbell},
  journal={Nature},
  year={2000},
  volume={407},
  pages={86-90}
}
Since the first report of live mammals produced by nuclear transfer from a cultured differentiated cell population in 1995 (ref. 1), successful development has been obtained in sheep, cattle, mice and goats using a variety of somatic cell types as nuclear donors. The methodology used for embryo reconstruction in each of these species is essentially similar: diploid donor nuclei have been transplanted into enucleated MII oocytes that are activated on, or after transfer. In sheep and goat pre… 
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TLDR
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TLDR
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TLDR
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Bovine somatic cell nuclear transfer.
TLDR
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Cats cloned from fetal and adult somatic cells by nuclear transfer.
TLDR
The cloned cats showed genotypes identical to the donor cell lines, indicating that adult somatic cells can be used for feline cloning.
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TLDR
Rabbit oocytes can be served as recipient cytoplasm for donor cells from different kinds of animals and showed that the donor cell type can significantly affect the in vitro development of cloned rabbit embryos.
Rabbits generated from fibroblasts through nuclear transfer.
TLDR
The present study demonstrates that rabbits can be cloned from adult fibroblasts after culture and shows that nuclear transfer embryos constructed using serum-starved fibro Blasts showed a significantly higher developmental rate than non-starred fibroBlasts through preimplantation stages.
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References

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TLDR
This is the first report, to the authors' knowledge, of live mammalian offspring following nuclear transfer from an established cell line, and will provide the same powerful opportunities for analysis and modification of gene function in livestock species that are available in the mouse through the use of embryonic stem cells.
Production of goats by somatic cell nuclear transfer
TLDR
Analysis of the milk of one of the transgenic cloned animals showed high-level production of human antithrombin III, similar to the parental transgenic line.
Production of cloned lambs from an established embryonic cell line: a comparison between in vivo- and in vitro-matured cytoplasts.
TLDR
DNA microsatellite markers conclusively show that the three lambs are genetically identical and were derived from the embryonic cell line, and some cells from this blastocyst-derived embryonic cellline are totipotent by nuclear transfer and can produce viable offspring.
Somatic cell nuclear transfer in mammals: progress and applications.
TLDR
The birth of sheep, cows, goats, and mice using this technique with fetal or adult cell donors is now established fact, and the success with adult mammalian cell donors extends the historic work in frogs by attesting to the totipotency of nuclei in at least some adult, differentiated cell types.
Full-term development of mice from enucleated oocytes injected with cumulus cell nuclei
TLDR
These experiments show that for mammals, nuclei from terminally differentiated, adult somatic cells of known phenotype introduced into enucleated oocytes are capable of supporting full development.
Production of gene-targeted sheep by nuclear transfer from cultured somatic cells
TLDR
Efficient and reproducible gene targeting in fetal fibroblasts to place a therapeutic transgene at the ovine α1(I) procollagen (COL1A1) locus is described and the production of live sheep by nuclear transfer is described.
Production of identical sextuplet mice by transferring metaphase nuclei from four-cell embryos.
  • O. Kwon, T. Kono
  • Biology
    Proceedings of the National Academy of Sciences of the United States of America
  • 1996
TLDR
To obtain embryos with diploid sets of chromosomes, nuclei from each reconstructed embryo were transferred individually into separate enucleated fertilized one-cell embryos, thus doubling the number of identical embryos.
Production of cloned calves following nuclear transfer with cultured adult mural granulosa cells.
TLDR
Adult somatic cell nuclear transfer was used to determine the totipotent potential of cultured mural granulosa cells, obtained from a Friesian dairy cow of high genetic merit, and DNA analyses confirmed that the calves are all genetically identical to the donor cow.
Nuclear transplantation in early pig embryos.
TLDR
Nuclear transfer was evaluated in early porcine embryos and a single piglet was born after the transfer of a 4-cell nucleus to an enucleated, activated metaphase II oocyte and subsequent in vivo development.
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