• DOI:10.1038/35024082
• Corpus ID: 4373081

Cloned pigs produced by nuclear transfer from adult somatic cells

@article{Polejaeva2000ClonedPP,
  title={Cloned pigs produced by nuclear transfer from adult somatic cells},
  author={Irina A. Polejaeva and Shu-Hung Chen and Todd D. Vaught and Raymond L. Page and J. I. Mullins and Suyapa F Ball and Yifan Dai and Jeremy Boone and Shawn Walker and David Ayares and Alan Colman and Keith Henry Stockman Campbell},
  journal={Nature},
  year={2000},
  volume={407},
  pages={86-90}
}

• I. Polejaeva, Shu-Hung Chen, +9 authors K. Campbell
• Published 7 September 2000
• Biology
• Nature

Since the first report of live mammals produced by nuclear transfer from a cultured differentiated cell population in 1995 (ref. 1), successful development has been obtained in sheep, cattle, mice and goats using a variety of somatic cell types as nuclear donors. The methodology used for embryo reconstruction in each of these species is essentially similar: diploid donor nuclei have been transplanted into enucleated MII oocytes that are activated on, or after transfer. In sheep and goat pre-activated oocytes have also proved successful as cytoplast recipients. The reconstructed embryos are then cultured and selected embryos transferred to surrogate recipients for development to term. In pigs, nuclear transfer has been significantly less successful; a single piglet was reported after transfer of a blastomere nucleus from a four-cell embryo to an enucleated oocyte; however, no live offspring were obtained in studies using somatic cells such as diploid or mitotic fetal fibroblasts as nuclear donors. The development of embryos reconstructed by nuclear transfer is dependent upon a range of factors. Here we investigate some of these factors and report the successful production of cloned piglets from a cultured adult somatic cell population using a new nuclear transfer procedure.