Cloned pigs produced by nuclear transfer from adult somatic cells

  title={Cloned pigs produced by nuclear transfer from adult somatic cells},
  author={Irina A. Polejaeva and Shu-Hung Chen and Todd D. Vaught and Raymond L. Page and J. I. Mullins and Suyapa F Ball and Yifan Dai and Jeremy Boone and Shawn Walker and David Ayares and Alan Colman and Keith Henry Stockman Campbell},
Since the first report of live mammals produced by nuclear transfer from a cultured differentiated cell population in 1995 (ref. 1), successful development has been obtained in sheep, cattle, mice and goats using a variety of somatic cell types as nuclear donors. The methodology used for embryo reconstruction in each of these species is essentially similar: diploid donor nuclei have been transplanted into enucleated MII oocytes that are activated on, or after transfer. In sheep and goat pre… 
Animal Cloning : Reprogramming the Donor Genome
Success rates remain low in all species, with published data showing that only 1% to 5% of reconstructed embryos result in l i ve b i r t hs ( see Ros l i n I ns t i t u te web s i t e).
Cloned pigs derived from somatic cell nuclear transfer embryos cultured in vitro at low oxygen tension
Results indicate that the use of in vitro matured oocytes from prepubertal gilts as recipient, combined with cloned embryos cultured at low oxygen tension is an effective way to produce cloned pigs.
Development of reconstituted embryos derived from somatic cell nuclei in the rabbit
This study examined to produce reconstituted embryos derived from somatic cell nuclei, and to establish embryonic stem (ES) cell lines from the embryo in rabbits to establish the ES cell lines derived from the embryos reconst ituted by nuclear transfer.
Nuclear Transfer in Rabbits with Different Types of Donor Cells
Results from the present study show that embryos cloned from embryonic cells had higher developmental potential than those cloning from somatic cells, and will open a new way to more wide use of rabbit model for basic and applied research.
Stem Cells and Cloning
Although the therapeutic promise is very great, the safety of transplanted cloned cells must be assessed, and the creation of in vitro cellular models of disease by therapeutic cloning will provide an exceptional tool for research and drug screening.
Bovine somatic cell nuclear transfer.
A basic protocol for bovine SCNT is described, currently being used in the laboratory, which is amenable for the use of the nuclear transplantation technique for research or commercial purposes.
Cats cloned from fetal and adult somatic cells by nuclear transfer.
The cloned cats showed genotypes identical to the donor cell lines, indicating that adult somatic cells can be used for feline cloning.
Development of Somatic Cell Nuclear Transfer Technique for Biomedical and Agricultural Research (year II)
Rabbit oocytes can be served as recipient cytoplasm for donor cells from different kinds of animals and showed that the donor cell type can significantly affect the in vitro development of cloned rabbit embryos.
Rabbits generated from fibroblasts through nuclear transfer.
The present study demonstrates that rabbits can be cloned from adult fibroblasts after culture and shows that nuclear transfer embryos constructed using serum-starved fibro Blasts showed a significantly higher developmental rate than non-starred fibroBlasts through preimplantation stages.


Sheep cloned by nuclear transfer from a cultured cell line
This is the first report, to the authors' knowledge, of live mammalian offspring following nuclear transfer from an established cell line, and will provide the same powerful opportunities for analysis and modification of gene function in livestock species that are available in the mouse through the use of embryonic stem cells.
Production of goats by somatic cell nuclear transfer
Analysis of the milk of one of the transgenic cloned animals showed high-level production of human antithrombin III, similar to the parental transgenic line.
Production of cloned lambs from an established embryonic cell line: a comparison between in vivo- and in vitro-matured cytoplasts.
DNA microsatellite markers conclusively show that the three lambs are genetically identical and were derived from the embryonic cell line, and some cells from this blastocyst-derived embryonic cellline are totipotent by nuclear transfer and can produce viable offspring.
Somatic cell nuclear transfer in mammals: progress and applications.
The birth of sheep, cows, goats, and mice using this technique with fetal or adult cell donors is now established fact, and the success with adult mammalian cell donors extends the historic work in frogs by attesting to the totipotency of nuclei in at least some adult, differentiated cell types.
Full-term development of mice from enucleated oocytes injected with cumulus cell nuclei
These experiments show that for mammals, nuclei from terminally differentiated, adult somatic cells of known phenotype introduced into enucleated oocytes are capable of supporting full development.
Production of gene-targeted sheep by nuclear transfer from cultured somatic cells
Efficient and reproducible gene targeting in fetal fibroblasts to place a therapeutic transgene at the ovine α1(I) procollagen (COL1A1) locus is described and the production of live sheep by nuclear transfer is described.
Production of identical sextuplet mice by transferring metaphase nuclei from four-cell embryos.
  • O. Kwon, T. Kono
  • Biology
    Proceedings of the National Academy of Sciences of the United States of America
  • 1996
To obtain embryos with diploid sets of chromosomes, nuclei from each reconstructed embryo were transferred individually into separate enucleated fertilized one-cell embryos, thus doubling the number of identical embryos.
Production of cloned calves following nuclear transfer with cultured adult mural granulosa cells.
Adult somatic cell nuclear transfer was used to determine the totipotent potential of cultured mural granulosa cells, obtained from a Friesian dairy cow of high genetic merit, and DNA analyses confirmed that the calves are all genetically identical to the donor cow.
Nuclear transplantation in early pig embryos.
Nuclear transfer was evaluated in early porcine embryos and a single piglet was born after the transfer of a 4-cell nucleus to an enucleated, activated metaphase II oocyte and subsequent in vivo development.