Cloned pigs generated from cultured skin fibroblasts derived from a H-transferase transgenic boar.

@article{Bondioli2001ClonedPG,
  title={Cloned pigs generated from cultured skin fibroblasts derived from a H-transferase transgenic boar.},
  author={K. Bondioli and Jagdeece Ramsoondar and Brent J. Williams and Cristina Chuva Costa and William Fodor},
  journal={Molecular reproduction and development},
  year={2001},
  volume={60 2},
  pages={189-95}
}
Cloned pigs were produced from cultured skin fibroblasts derived from a H-transferase transgenic boar. One 90 day fetus and two healthy piglets resulted from nuclear transfer by fusion of cultured fibroblasts with enucleated oocytes. The cells used in these studies were subjected to an extensive culture time, freezing and thawing, and clonal expansion from single cells prior to nuclear transfer. PCR and FACS analysis determined that the cloned offspring contained and expressed the H-transferase… CONTINUE READING

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