Cloned fragment A of diphtheria toxin is expressed and secreted into the periplasmic space of Escherichia coli K12.

Abstract

An 831-base pair segment of the corynebacteriophage beta tox-45 genome encoding fragment A of diphtheria toxin was cloned into plasmid pUC8 in Escherichia coli K12. Strains containing recombinant plasmids expressed the adenosine diphosphate ribosyl transferase activity characteristic of fragment A; this activity could be inhibited by polyvalent antiserum to… (More)

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@article{Leong1983ClonedFA, title={Cloned fragment A of diphtheria toxin is expressed and secreted into the periplasmic space of Escherichia coli K12.}, author={Dan Leong and Kenneth D. Coleman and John Murphy}, journal={Science}, year={1983}, volume={220 4596}, pages={515-7} }