Cloned cytosine deaminase gene expression of Bifidobacterium longum and application to enzyme/pro-drug therapy of hypoxic solid tumors.

@article{Nakamura2002ClonedCD,
  title={Cloned cytosine deaminase gene expression of Bifidobacterium longum and application to enzyme/pro-drug therapy of hypoxic solid tumors.},
  author={Toshiyuki Nakamura and Takayuki Sasaki and Minoru Fujimori and Kazuyuki Yazawa and Yasunobu Kano and Jun Amano and Shun'ichiro Taniguchi},
  journal={Bioscience, biotechnology, and biochemistry},
  year={2002},
  volume={66 11},
  pages={2362-6}
}
Bifidobacterium longum is a nonpathogenic anaerobic bacterium among normal bacterial flora. Recently, it was reported that B. longum accumulated in hypoxic solid tumors. The gene of interest was expressed in transfected B. longum by the shuttle vector pBLES100 in solid tumors. In this report, we constructed pBLES100-S-eCD, which included the cytosine deaminase gene. We confirmed by western blotting that transfected B. longum produced cytosine deaminase. In addition, transfected B. longum… CONTINUE READING