Cloned and expressed fungal phyA gene in alfalfa produces a stable phytase.

@article{Ullah2002ClonedAE,
  title={Cloned and expressed fungal phyA gene in alfalfa produces a stable phytase.},
  author={Abul H. J. Ullah and Kandan Sethumadhavan and Edward J. Mullaney and Thomas Ziegelhoffer and Sandra Austin-Phillips},
  journal={Biochemical and biophysical research communications},
  year={2002},
  volume={290 4},
  pages={1343-8}
}
The phyA gene from Aspergillus ficuum that codes for a 441-amino-acid full-length phosphomonoesterase (phytase) was cloned and expressed in Medicago sativa (alfalfa) leaves. The expressed enzyme from alfalfa leaves was purified to homogeneity and biochemically characterized, and its catalytic properties were elucidated. The expressed phytase in alfalfa leaves retained all the biochemical properties of the benchmark A. ficuum phytase. Although the characteristic bi-hump pH optima were retained… CONTINUE READING
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