Clonal structure of Shiga toxin (Stx)-producing and beta-D-glucuronidase-positive Escherichia coli O157:H7 strains isolated from outbreaks and sporadic cases in Hokkaido, Japan.

@article{Nagano2002ClonalSO,
  title={Clonal structure of Shiga toxin (Stx)-producing and beta-D-glucuronidase-positive Escherichia coli O157:H7 strains isolated from outbreaks and sporadic cases in Hokkaido, Japan.},
  author={Hideki Nagano and Toyo Okui and Osamu Fujiwara and Yasuhiro Uchiyama and Naoto Tamate and Hiroyuki Kumada and Yo Morimoto and Shoki Yano},
  journal={Journal of medical microbiology},
  year={2002},
  volume={51 5},
  pages={405-16}
}
A total of 22 clonal phenotypic variants of Shiga toxin (Stx)-producing Escherichia coli (STEC) O157:H7 was isolated from six different locations in Hokkaido, Japan. These isolates were negative for sorbitol fermentation but positive for beta-D-glucuronidase (GUD+). They carried eaeA, EHEC-hlyA, pas and etpD genes like typical E. coli O157:H7 and, in addition, st1 and stx2 genes. However, they were shown to lack katP and espP genes that are present in typical STEC O157:H7. All these atypical… CONTINUE READING

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