Clonal selection and in vivo quantitation of protein interactions with protein-fragment complementation assays.

@article{Remy1999ClonalSA,
  title={Clonal selection and in vivo quantitation of protein interactions with protein-fragment complementation assays.},
  author={Ingrid Remy and Stephen W. Michnick},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1999},
  volume={96 10},
  pages={5394-9}
}
Two strategies are described for detecting constitutive or induced protein-protein interactions in intact mammalian cells; these strategies are based on oligomerization domain-assisted complementation of rationally designed fragments of the murine enzyme dihydrofolate reductase (DHFR; EC 1.5.1.3). We describe a dominant clonal-selection assay of stably transfected cells expressing partner proteins FKBP (FK506 binding protein) and FRAP (FKBP-rapamycin binding protein) fused to DHFR fragments and… CONTINUE READING

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Remy and Michnick Proc

J. Zhang, Lautar, S. Anal. Biochem. 242, 68–72. Biochemistry
Natl. Acad. Sci. USA 96 • 1999
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