Clonal propagation and the fast generation of karyotype diversity: an in vitro Leishmania model

@article{Dujardin2006ClonalPA,
  title={Clonal propagation and the fast generation of karyotype diversity: an in vitro Leishmania model},
  author={Jean-Claude Dujardin and Simonne de Doncker and Diane Jacquet and Anne-Laure Ba{\~n}uls and M Balavoine and Dirk R. van Bockstaele and Michel Tibayrenc and Jorge Arevalo and Dominique Le Ray},
  journal={Parasitology},
  year={2006},
  volume={134},
  pages={33 - 39}
}
In the present work we studied the karyotype stability during long-term in vitro maintenance in 3 cloned strains of Leishmania (Viannia) peruviana, Leishmania (Viannia) braziliensis and a hybrid between both species. Only the L. (V.) peruviana strain showed an unstable karyotype, even after subcloning. Four chromosomes were studied in detail, each of them characterized by homologous chromosomes of different size (heteromorphy). Variations in chromosome patterns during in vitro maintenance were… 
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References

SHOWING 1-10 OF 42 REFERENCES
Molecular epidemiology and diagnosis of Leishmania: what have we learnt from genome structure, dynamics and function?
Plasticity in chromosome number and testing of essential genes in Leishmania by targeting.
TLDR
The results show that virulent Leishmania require at least one copy of dhfr-ts and argue that DHFR-TS plays an unanticipated role in addition to its role in the de novo synthesis of thymidine.
Karyotype polymorphism and conserved characters in the Leishmania (Viannia) braziliensis complex explored with chromosome-derived probes.
TLDR
Molecular karyotype of 45 reference populations of Neotropical leishmanias was analyzed and size-conserved patterns were identified and found to be specific to subgenus Viannia and to its constitutive species.
Effect of large targeted deletions on the mitotic stability of an extra chromosome mediating drug resistance in Leishmania.
TLDR
A mitotically stable linear extra chromosome obtained in a Leishmania donovani strain rendered mycophenolic acid-resistant has been physically mapped and its enrichment in homopolymer tracts suggests that this region might contain an origin of replication.
Size-conserved chromosomes and stability of molecular karyotype in cloned stocks of Leishmania major.
Leishmania: sex, lies and karyotype.
Plasticity of gp63 gene organization in Leishmania (Viannia) braziliensis and Leishmania (Viannia) peruviana.
TLDR
The genomic organization of gp63 genes in 4 and 7 isolates of Leishmania braziliensis and L. peruviana was studied by RFLP analysis with 3 restriction enzymes and showed a marked polymorphism among isolates, which may suggest the existence of at least 2 arrays of heterologous gp63 repeats.
Size-polymorphism of mini-exon gene-bearing chromosomes among natural populations of Leishmania, subgenus Viannia.
Is Leishmania (Viannia) peruviana a Distinct Species? A MLEE/RAPD Evolutionary Genetics Answer
Abstract A set of 38 Leishmania stocks from the Andean valleys of Peru was characterized by both Multilocus Enzyme Electrophoresis (MLEE) and Random Amplified Polymorphic DNA (RAPD). Data were…
Karyotype plasticity in neotropical Leishmania: an index for measuring genomic distance among L. (V.) peruviana and L. (V.) braziliensis populations.
TLDR
The Chromosome Size Difference Index evidenced the nature of relationships between L. braziliensis and L. peruviana and it provided a coherent picture of geographical and genomic differentiation among parasite populations.
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