• Medicine
  • Published in Bone marrow transplantation 1992

Clonal imbalances of serum immunoglobulins after allogeneic bone marrow transplantation: an analysis by high-resolution two-dimensional gel electrophoresis.

@article{Tissot1992ClonalIO,
  title={Clonal imbalances of serum immunoglobulins after allogeneic bone marrow transplantation: an analysis by high-resolution two-dimensional gel electrophoresis.},
  author={J D Tissot and Claudine Helg and Bernard Chapuis and Rudolf H. Zubler and Michel Jeannet and Denis F. Hochstrasser and Patrick Hohlfeld and Paula Schneider},
  journal={Bone marrow transplantation},
  year={1992},
  volume={10 4},
  pages={
          347-53
        }
}
The clonality pattern of immunoglobulins (Igs) produced after allogeneic bone marrow transplantation (BMT) was studied by high-resolution two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) of serum samples and purified Igs. With this technique, the light (L) chain of a monoclonal Ig usually appears as a single spot. Thus, the degree of clonal diversity of the functional B cells can be appreciated by the electrophoretic pattern of the serum L chains. Furthermore, 2D-PAGE allows a semi… CONTINUE READING