Clonal growth of primary cultures of human hyaline chondrocytes in a defined medium.

@article{Jennings1983ClonalGO,
  title={Clonal growth of primary cultures of human hyaline chondrocytes in a defined medium.},
  author={S. M. Jennings and Richard G. Ham},
  journal={Cell biology international reports},
  year={1983},
  volume={7 2},
  pages={149-59}
}
Rapid clonal growth of primary cultures of human costal chondrocytes in a defined medium has been achieved. The basal nutrient medium used for such growth is MCDB 104. It is prepared without linoleic acid and supplemented with 1 microgram/ml insulin, 100 ng/ml fibroblast growth factor, 1.0 x 10(-7) M dexamethasone, and 5 micrograms/ml mixed lipids, presented to the cells in the form of liposomes. The lipid supplement contains soybean lecithin, cholesterol, sphingomyelin, vitamin E, and vitamin… CONTINUE READING